[Termination of Replication and Mechanisms of Heteroplasmy in Sturgeon Mitochondrial DNA].

The control region of mitochondrial DNA (mtDNA) in sturgeon contains one to seven tandem nucleotide repeats 78-83 bp in size. Some sturgeon species are homoplasmic by the D-loop size (Acipenser nudiventris, A. oxyrinchus, A. sturio), some are mildly heteroplasmic (A. fulvescens, Huso huso) and some are markedly heteroplasmic (A. brevirostrum, A. medirostris, A. mikadoi, A. naccarii, and A. transmontanus). This work presents a comparison of the D-loop sequences associated with the termination of mtDNA replication in fish and the conservative sequences determining the termination of replication (TAS) in these organisms. It is proposed that the D-loop heteroplasmy in sturgeon may be associated with variation in the number of tandem repeat sequences, which can form stable spatial structures during mtDNA replication. In most sturgeon species with pronounced heteroplasmy, the energy levels required for the folding of tandem repeats containing variable number of repeated units differ minimally.

[Features of redox processes in the amniotic fluid at placental insufficiency]. / Osobennosti okislitel'no-vosstanovitel'nykh protsessov v okoloplodnykh vodakh pri platsentarnoi nedostatochnosti.

Activity of prooxidant enzymes (NADPH-oxidase and xanthine oxidase), antioxidant enzymes (superoxide dismutase (SOD) and catalase), enzymes of the glutathione-dependent systems, as well as antioxidant vitamins (retinol and a-tocopherol), lipid peroxidation products (LPP) (conjugated dienes and Schiff bases), and peroxide chemiluminescence were studied in the amniotic fluid at different periods of physiological pregnancy and placental insufficiency (PI). It was found that at PI the activity of NADPH-oxidase, xanthine oxidase increased and the activity of SOD, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase and the content of fat-soluble vitamins decreased. The direct and inverse correlation between the studied pro- and antioxidant parameters and the content of LPP products, was found ro be different in the II and III trimesters of gestation. The revealed differences obviously reflect metabolic impairments in the fetoplacental complex, and the activity and level of the paremeters of redox processes can be used as tests for pre- and postnatal disorders.

MicroRNA-Dependent Regulation of IGF1R Gene Expression in Hormone-Sensitive and Hormone-Resistant Prostate Cancer Cells.

Using multiple parallel sequencing on Illumina platform, we identified eight microRNAs that showed significant opposite changes of gene expression in cells of the hormone-sensitive LNCaP prostate cancer cell line and in cells of the hormone-resistant DU-145 cell line, in comparison to the microRNA expression in the normal prostate tissue cells. We found that the insulin-like growth factor 1 receptor (IGF1R) gene is a target of five microRNAs whose expression is increased in LNCaP cells and reduced in DU-145 cells.

[Comparative assessment of dot-immunoassay and immunochromatography methods for detection of 01 serogroup of vibrio cholerae].

AIM: Comparative study of sensitivity and specificity of immunochromatographic (IC) assay kit and dot-immunoanalysis for assessment of feasibility of their use for laboratory diagnostics of cholera. MATERIALS AND METHODS: Experimental lots of IC assay kit and dot-immunoassay (DIA) for detection of Vibrio cholerae serogroup 01 serovars Ogava and Inaba were constructed on the basis of species-specific monoclonal antibodies (MCA) conjugated with colloid gold (IC) and peroxidase (DIA). Hybridoma-producer of MCAwas obtained and stored in liquid nitrogen in Rostov-on-Don Research Institute for Plague Control. It was deposited in specialized collection of cell cultures of vertebrates in Institute of Cytology (Saint Petersburg). RESULTS: Strong specificity of IC assay kit and DIA relative to cholera vibrios 01 and absence of crossreactivity with closely related and heterologous microorganisms were shown. Minimal quantity of vibrios, which could be detected using IC assay kit and DIA, was 107 and 105-106 microbial cells respectively. CONCLUSION: Performance of IC assay takes 5-15 min, DIA--1.5 hour, they allow to visually assess the reaction, do not require instrumentation and in perspective both methods could be used on defined stages of scheme for laboratory analysis of cholera.